Isolation and Purification of Protein Granules from Escherichia coli Cells Overproducing Bovine Growth Hormone


High-level expression of bovine growth hormone in Escherichia coli results in the formation of distinct cytoplasmic granules that are visible with the phase-contrast microscope. These granules were examined by transmission and scanning electron microscopy. Intact granules were isolated from crude cell lysates by differential centrifugation and were further purified by a simple washing procedure that yields nearly homogeneous bovine growth hormone.

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Schoner, R., Ellis, L. & Schoner, B. Isolation and Purification of Protein Granules from Escherichia coli Cells Overproducing Bovine Growth Hormone. Nat Biotechnol 3, 151–154 (1985).

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