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One photon up, one photon down

A microscopy technique based on stimulated Raman scattering achieves label-free imaging with very high sensitivity.

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Figure 1: Before passing through the sample, the pump laser (blue) is unmodulated and the probe laser (red) is modulated, that is, its amplitude is varied with a high frequency.

Kim Caesar

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Gratton, E., Digman, M. One photon up, one photon down. Nat Biotechnol 27, 147–148 (2009). https://doi.org/10.1038/nbt0209-147

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