Abstract
We describe the application of robotics to the purification and isolation of synthetic oligodeoxyribonucleotides. Starting with commercially available components, we have designed and programmed a robot based system that can isolate oligonucleotides from polyacrylamide gel slices, prepare oligonucleotides for purification by polyacrylamide gel electrophoresis and execute a rapid purification protocol on a disposable Cā18 reverse phase column. Each operation has been fully automated, including evaporative centrifugation, such that the system can execute any two procedures simultaneously with a throughput of 12 samples in about six hours.
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Jones, S., Brown, J., Vanstone, D. et al. Automating the Purification and Isolation of Synthetic DNA. Nat Biotechnol 5, 67ā70 (1987). https://doi.org/10.1038/nbt0187-67
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DOI: https://doi.org/10.1038/nbt0187-67