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Automating the Purification and Isolation of Synthetic DNA

Abstract

We describe the application of robotics to the purification and isolation of synthetic oligodeoxyribonucleotides. Starting with commercially available components, we have designed and programmed a robot based system that can isolate oligonucleotides from polyacrylamide gel slices, prepare oligonucleotides for purification by polyacrylamide gel electrophoresis and execute a rapid purification protocol on a disposable Cā€“18 reverse phase column. Each operation has been fully automated, including evaporative centrifugation, such that the system can execute any two procedures simultaneously with a throughput of 12 samples in about six hours.

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Jones, S., Brown, J., Vanstone, D. et al. Automating the Purification and Isolation of Synthetic DNA. Nat Biotechnol 5, 67ā€“70 (1987). https://doi.org/10.1038/nbt0187-67

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