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Direct Enzymatic Synthesis of Penicillin G Using Cyclases of Penicillium chrysogenum and Acremonium chrysogenum


Direct enzymatic synthesis of penicillin G has been obtained by in vitro cyclization of the linear tripeptide–like molecule phenyl–acetyl–L–cysteinyl–D–valine (PCV) using partially purified cyclases of Penicillium chrysogenum and Acremonium chrysogenum. The reaction product shows an antibiotic spectrum similar to that of penicilin G, is sensitive to β–lactamase and penicillin acylase, and has been identified by chromatography, electrophoresis and HPLC as penicillin G.

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Luengo, J., Alemany, M., Salto, F. et al. Direct Enzymatic Synthesis of Penicillin G Using Cyclases of Penicillium chrysogenum and Acremonium chrysogenum. Nat Biotechnol 4, 44–47 (1986).

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