Research Paper | Published:

Potential Shuttle Vectors Based on the Methanogen Plasmid pME2001

Bio/Technologyvolume 3pages6972 (1985) | Download Citation



Methane is produced by anaerobic archaebacteria known as methanogens. Currently the only available plasmid from a methanogen is pME2001. We have incorporated pME2001 into plasmids which should be capable of replication in a range of microbial host species. Plasmid pET2411, a recombinant plasmid formed by joining pBR322 to pME2001, directs the synthesis of pME2001 encoded polypeptides in Escherichia coli but cannot replicate in E. coli in the absence of E. coli DNA polymerase I.

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  1. Department of Microbiology, The Ohio State University, Columbus, Ohio, 43210

    • Leo Meile
    •  & John N. Reeve


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