Research Paper | Published:

Ethanol Accumulation in Cultures of Pachysolen Tannophilus on D-Xylose is Associated with a Transition to a State of Low Oxygen Consumption

Bio/Technologyvolume 3pages5962 (1985) | Download Citation

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Abstract

Ethanol accumulation in aerobic batch cultures of Pachysolen tannophilus growing on D-xylose is associated with a transition to a state of low oxygen consumption, and the events triggering the accumulation differ from those of a Crabtree effect. The transition explains the accumulation of ethanol at only a particular interval during the growth of the culture, shows that such accumulation is not dependent on limited oxygen supply, and offers approaches to enhancing the yield of ethanol.

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References

  1. 1

    Rydholm, S.A. 1965. Pulping Processes, p. 97. Interscience Publishers, New York.

  2. 2

    Maleszka, R., Veliky, I.A. and Schneider, H. 1981. Enhanced rate of ethanol production from D-xylose using recycled or immobilized cells of Pachysolen tannophilus. Biotechnol. Lett. 3: 415–420.

  3. 3

    Slininger, P.J., Bothast, R.J., Van Cauwenberge, J.E. and Kurtzman, C.P. 1982. Conversion of D-xylose to ethanol by the yeast Pachysolen tannophilus. Biotechnol. and Bioeng. 24: 371–384.

  4. 4

    du Preez, J.C. and van der Walt, J.P. 1983. Fermentation of D-xylose to ethanol by a strain of Candida shehatae. Biotechnol. Lett. 5: 357–362.

  5. 5

    Slapack, G., Edey, E., Mahmourides, G. and Schneider, H. 1984. Ethanol from hexoses and pentoses in spent sulfite liquor. In: Biotechnology Advances, M. Moo-Young, (ed.), Pergamon Press. In press.

  6. 6

    Margaritis, A. and Bajpai, P. 1982. Direct fermentation of D-xylose to ethanol by Kluyveromyces marxianus strains. Appl. Environ. Microbiol. 44: 1039–1041.

  7. 7

    Gong, C.-S., McCracken, L.D. and Tsao, G.T. 1981. Direct fermentation of D-xylose to ethanol by a xylose-fermenting yeast mutant, Candida sp. XF217. Biotechnol. Lett. 3: 245–250.

  8. 8

    Fietcher, A., Fuhrmann, G.F. and Kappeli, O. 1981. Regulation of glucose metabolism in growing yeast cells. Adv. Microbiol. Physiol. 22: 123–183.

  9. 9

    Maleszka, R. and Schneider, H. 1982. Fermentation of D-xylose, xylitol and D-xylulose by yeasts. Can. J. Microbiol. 28: 360–363.

  10. 10

    Schneider, H., Maleszka, R., Neirinck, L., Veliky, I.A., Wang, P.Y. and Chan, Y.K. 1983. Ethanol production from D-xylose and several other carbohydrates by Pachysolen tannophilus and other yeasts. Adv. in Biochem. Engineering/Biotechnology 27: 57–71.

  11. 11

    du Preez, J.C., Prior, B.A. and Monteiro, A.M.T. 1984. The effect of aeration on xylose fermentation by Candida shehatae and Pachysolen tannophilus. Arch. Microbiol. Biotechnol. 19: 261–266.

  12. 12

    Baillargeon, M.W., Jansen, N.B., Gong, C.S. and Tsao, G.T. 1983. Effect of oxygen uptake rate on ethanol production by a xylose-fermenting yeast mutant, Candida sp. XF217. Biotechnol. Lett. 5: 339–344.

  13. 13

    Jeffries, T.W. 1981. Conversion of xylose to ethanol under aerobic conditions by Candida tropicalis. Biotechnol. Lett. 3: 213–218.

  14. 14

    Schvester, P., Robinson, C.W. and Moo-Young, M. 1983. Xylose fermentation to ethanol by Pachysolen tannophilus. Biotech. Bioeng. Symp. 13: 131–152.

  15. 15

    Debus, D., Methner, H., Schulze, D. and Dellweg, H. 1983. Fermentation of xylose with the yeast Pachysolen tannophilus. Eur. J. Appl. Microbiol. Technol. 17: 287–291.

  16. 16

    Maleszka, R. and Schneider, H. 1982. Concurrent production and consumption of ethanol by cultures of Pachysolen tannophilus growing on D-xylose. Appl. Environ. Microbiol. 44: 909–912.

  17. 17

    Mahmourides, G., Maleszka, R., James, A.P. and Schneider, H. 1983. Mutants of Pachysolen tannophilus that produce enhanced amounts of acetic acid from D-xylose and other sugars. Biotechnol. Lett. 5: 29–32.

  18. 18

    Woehrer, W. and Roehr, M. 1981. Regulatory aspects of bakers' yeast metabolism in aerobic fed-batch cultures. Biotechnol. Bioeng. 23: 567–581.

  19. 19

    Magdalena, P., Kappeli, O. and Fiechter, A. 1983. An expanded concept for the glucose effect in the yeast Saccharomyces cerevisiae. Involvement of short- and long-term regulation. J. Gen. Microbiol. 129: 43–49.

  20. 20

    Utter, M.F., Duell, E.A. and Bernofsky, C. 1968. Alterations in the respiratory enzymes of the mitochondria of growing and resting yeast, p. 197–215. In: Aspects of Yeast Metabolism, A. K. Mills and H. Krebs (eds.), Blackwell Scientific Publications, Oxford and Edinburgh.

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Author notes

  1. George Mahmourides, Hung Lee, Nancy Maki and Henry Schneider: Issued as NRCC publication number 23752.

Affiliations

  1. Division of Biological Sciences, National Research Council of Canada, Ottawa, Canada, K1A OR6

    • George Mahmourides
    • , Hung Lee
    • , Nancy Maki
    •  & Henry Schneider

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DOI

https://doi.org/10.1038/nbt0185-59

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