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Genome resequencing and genetic variation

Nature Biotechnology volume 26, pages 65–66 (2008)Cite this article

Technologies for selecting segments of large genomes for resequencing will reveal biologically important sequence variation.

Resequencing the genome of many individuals for which there is a reference genome allows investigation of the relationship between sequence variation and normal or disease phenotypes. Recent technological advances in shotgun (that is, randomly directed) DNA sequencing have substantially increased the power to harvest variation5,6. Some of these platforms permit ∼100-fold greater rates of DNA sequencing at approximately 100-fold lower cost per base. But even these technologies will not realistically allow the resequencing of more than a few human genomes in the near future. However, if this new sequencing power could be targeted to limited areas of large genomes, it would become feasible to study variation in these regions in thousands of individuals. This configuration of the new sequencing technologies would allow direct and practical application to studies of human disease.

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References

  1. Albert, T.J. et al. Nat. Methods 4, 903–905 (2007).

    CAS  Article  Google Scholar 

  2. Okou, D.T. et al. Nat. Methods 4, 907–909 (2007).

    CAS  Article  Google Scholar 

  3. Porreca, G.J. et al. Nat. Methods 4, 931–936 (2007).

    CAS  Article  Google Scholar 

  4. Hodges, E. et al. Nat. Genet. 39, 1522–1527 (2007).

    CAS  Article  Google Scholar 

  5. Margulies, M. et al. Nature 437, 376–380 (2005).

    CAS  Article  Google Scholar 

  6. Bentley, D.R. Curr. Opin. Genet. Dev. 16, 545–552 (2006).

    CAS  Article  Google Scholar 

  7. Morgan, J.G. et al. Nucleaic Acids Res 20, 5173–5179 (1992).

    CAS  Article  Google Scholar 

Download references

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  1. Michael Stratton is at the Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK. mrs@sanger.ac.uk

    Michael Stratton

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  1. Michael Stratton
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Stratton, M. Genome resequencing and genetic variation. Nat Biotechnol 26, 65–66 (2008). https://doi.org/10.1038/nbt0108-65

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  • DOI: https://doi.org/10.1038/nbt0108-65

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