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Laser surgery for mouse geneticists

Nature Biotechnology volume 25, pages 59–60 (2007)Cite this article

A rapid, new approach for generating mutant mice from embryonic stem cells may accelerate high-throughput phenotype analysis.

The ability to select and identify embryonic stem (ES) cell clones with targeted and knockout mutations has revolutionized the use of the mouse as a model organism for studying human disease. To facilitate gene function assignment, worldwide efforts are underway to generate an ES cell resource with a knockout mutation in every gene in the mouse genome1,2. In this issue, Poueymirou et al.3 describe a procedure that has the potential to greatly increase the speed and efficiency of phenotyping mice made from ES cells.

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Figure 1: Comparison of homozygous mutant mouse production by conventional and laser-assisted microinjection procedures.

Kim Caesar

References

  1. Austin, C.P. et al. Nat. Genet. 36, 921–924 (2004).

    CAS  Article  Google Scholar 

  2. Auwerx, J. et al. Nat. Genet. 36, 925–927 (2004).

    CAS  Article  Google Scholar 

  3. Poueymirou, W.T. et al. Nat. Biotechnol. 25, 91–99 (2007).

    CAS  Article  Google Scholar 

  4. Eggan, K. et al. Nat. Biotechnol. 20, 455–459 (2002).

    CAS  Article  Google Scholar 

  5. Tokunaga, T. & Tsunoda, Y. Dev. Growth Differ. 34, 561–566 (1992).

    Article  Google Scholar 

  6. Mortensen, R.M., Conner, D.A., Chao, S., Geisterfer-Lowrance, A.A. & Seidman, J.G. Mol. Cell. Biol. 12, 2391–2395 (1992).

    CAS  Article  Google Scholar 

  7. Abuin, A. & Bradley, A. Mol. Cell. Biol. 16, 1851–1856 (1996).

    CAS  Article  Google Scholar 

  8. Brook, F.A. & Gardner, R.L. Proc. Natl. Acad. Sci. USA 94, 5709–5712 (1997).

    CAS  Article  Google Scholar 

  9. Schoonjans, L. et al. Stem Cells 21, 90–97 (2003).

    Article  Google Scholar 

Download references

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  1. Jackson Laboratory, Bar Harbor, 04609, Maine, USA

    Thomas Gridley & Rick Woychik

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  1. Thomas Gridley
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  2. Rick Woychik
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Gridley, T., Woychik, R. Laser surgery for mouse geneticists. Nat Biotechnol 25, 59–60 (2007). https://doi.org/10.1038/nbt0107-59

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  • DOI: https://doi.org/10.1038/nbt0107-59

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