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Preserving the genetic integrity of human embryonic stem cells

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This work was supported in part by funding provided by the Georgia Tech/Emory Center (GTEC) for the Engineering of Living Tissues, an ERC Program of the National Science Foundation under award no. EEC-9731643. This work was also supported in part by funding from the National Institutes of Health (NIH-HL074303). We thank Bresagen Inc., for providing the hES cells used in this correspondence, and Roger Nielsen for assistance with real-time PCR analysis.

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Correspondence to Steven L. Stice.

Supplementary information

Supplementary Fig. 1

G-banded karyotype of BG01 hES cells at p42 (mechanical) + p23 (non-enzymatic)

Supplementary Fig. 2

Fluorescence in situ hybridization signal patterns for cell line BG02 p12 (mechanical) + p56 (enzymatic)

Supplementary Table 1

Fold change values for individual genes across three populations

Supplementary Methods

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Mitalipova, M., Rao, R., Hoyer, D. et al. Preserving the genetic integrity of human embryonic stem cells. Nat Biotechnol 23, 19–20 (2005).

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