a) Representative images of placenta distribution (24 hours post treatment) of partially and fully modified Cy3-hsiRNAsFLT1-2283 (red) following 10 mg/kg IV or SC injections. Nuclei stained with DAPI (blue). Scale bar, 1mm. All images were acquired at identical settings (n=3 biologically independent animals). b) Guide strand quantification by PNA hybridization-based assay in placentas from a (n=6 biologically independent samples). Boxes represent 25 to 75 percentiles and whiskers indicate minimal and maximal values (****P<0.0001, **P=0.015, unpaired t test, two-tailed).