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Domestication of wild tomato is accelerated by genome editing

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Abstract

Crop improvement by inbreeding often results in fitness penalties and loss of genetic diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by using multiplex CRISPR–Cas9 editing of coding sequences, cis-regulatory regions or upstream open reading frames of genes associated with morphology, flower and fruit production, and ascorbic acid synthesis. Cas9-free progeny of edited plants had domesticated phenotypes yet retained parental disease resistance and salt tolerance.

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Change history

  • 05 October 2018

    In the version of this article initially published, the file with supplementary tables posted was from a different article. The correct file has now been posted.

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Acknowledgements

We thank W. Yang and N. Li (China Agricultural University) for providing the bacterial spot race T3 strain and for assistance with inoculation, and D. Bartlem (KWS) for critical reading and editing of an earlier draft of this manuscript. We also thank Y. Wang (Institute of Genetics and Developmental Biology, CAS) for help with Figure 1a. We thank L. Yan and Y. Li for assistance with tissue culture. This work was supported by grants from the National Key Research and Development Program of China (2016YFD0101804), the National Science Foundation of China (31788103 and 31420103912) and the Chinese Academy of Sciences (QYZDY-SSW-SMC030 and GJHZ1602) to C.G., and the Thousand Talents Plan to C.X.

Author information

Author notes

    • Tingdong Li
    • , Xinping Yang
    •  & Yuan Yu

    These authors contributed equally to this work.

Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

    • Tingdong Li
    • , Xiaomin Si
    • , Huawei Zhang
    •  & Caixia Gao
  2. University of Chinese Academy of Sciences, Beijing, China.

    • Tingdong Li
    • , Yuan Yu
    • , Xiaomin Si
    • , Caixia Gao
    •  & Cao Xu
  3. State Key Laboratory of Plant Genomics, National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

    • Xinping Yang
    • , Yuan Yu
    • , Xiawan Zhai
    • , Wenxia Dong
    •  & Cao Xu
  4. CAS-JIC Centre of Excellence for Plant and Microbial Science (CEPAMS), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

    • Xinping Yang
    • , Yuan Yu
    • , Xiawan Zhai
    • , Wenxia Dong
    •  & Cao Xu

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Contributions

C.X. and C.G. designed the experiments; T.L., X.Y., Y.Y. and X.S. performed most of the experiments; X.Z. and W.D. generated transgenic plants. T.L., X.Y., Y.Y., X.S. and H.Z. analyzed the results; C.X. and C.G. supervised the project; C.X. and C.G. wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Caixia Gao or Cao Xu.

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Figures 1–8

  2. 2.

    Life Sciences Reporting Summary

  3. 3.

    Supplementary Sequences

    The sequences of SP, SP5G, SlCLV3, SlWUS and SlGGP1 in S. pimpinellifolium (LA1589)

  4. 4.

    Supplementary Tables

    Supplementary Tables 1–8

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DOI

https://doi.org/10.1038/nbt.4273