Abstract
Crop improvement by inbreeding often results in fitness penalties and loss of genetic diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by using multiplex CRISPR–Cas9 editing of coding sequences, cis-regulatory regions or upstream open reading frames of genes associated with morphology, flower and fruit production, and ascorbic acid synthesis. Cas9-free progeny of edited plants had domesticated phenotypes yet retained parental disease resistance and salt tolerance.
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Change history
05 October 2018
In the version of this article initially published, the file with supplementary tables posted was from a different article. The correct file has now been posted.
References
Meyer, R.S. & Purugganan, M.D. Nat. Rev. Genet. 14, 840–852 (2013).
Østerberg, J.T. et al. Trends Plant Sci. 22, 373–384 (2017).
Zsögön, A., Cermak, T., Voytas, D. & Peres, L.E. Plant Sci. 256, 120–130 (2017).
Zuriaga, E. et al. Genet. Resour. Crop Evol. 56, 39–51 (2008).
Robbins, M.D., Darrigues, A., Sim, S.C., Masud, M.A. & Francis, D.M. Phytopathology 99, 1037–1044 (2009).
Rao, E.S. et al. Euphytica 190, 215–228 (2013).
Doebley, J.F., Gaut, B.S. & Smith, B.D. Cell 127, 1309–1321 (2006).
Huang, X. et al. Nature 537, 629–633 (2016).
Lin, T. et al. Nat. Genet. 46, 1220–1226 (2014).
Yin, K., Gao, C. & Qiu, J.L. Nat. Plants 3, 17107 (2017).
Rodríguez-Leal, D., Lemmon, Z.H., Man, J., Bartlett, M.E. & Lippman, Z.B. Cell 171, 470–480 e8 (2017).
Soyk, S. et al. Nat. Genet. 49, 162–168 (2017).
Park, S.J. et al. Nat. Genet. 46, 1337–1342 (2014).
Lippman, Z. & Tanksley, S.D. Genetics 158, 413–422 (2001).
van der Knaap, E. et al. Front. Plant Sci. 5, 227 (2014).
Xu, C. et al. Nat. Genet. 47, 784–792 (2015).
Endrizzi, K., Moussian, B., Haecker, A., Levin, J.Z. & Laux, T. Plant J. 10, 967–979 (1996).
Somssich, M., Je, B.I., Simon, R. & Jackson, D. Development 143, 3238–3248 (2016).
Cˇermák, T. et al. Plant Cell 29, 1196–1217 (2017).
Zhang, H.W. et al. Nat. Biotechnol. 36, 894–898 (2018).
Xing, H.L. et al. BMC Plant Biol. 14, 327 (2014).
Van Eck, J., Kirk, D.D. & Walmsley, A.M. Methods Mol. Biol. 343, 459–473 (2006).
Brooks, C., Nekrasov, V., Lippman, Z.B. & Van Eck, J. Plant Physiol. 166, 1292–1297 (2014).
Shan, Q., Wang, Y., Li, J. & Gao, C. Nat. Protoc. 9, 2395–2410 (2014).
Yang, W. & Francis, D.M. J. Am. Soc. Hortic. Sci. 130, 716–721 (2005).
Liu, H. et al. Mol. Plant 10, 530–532 (2017).
Acknowledgements
We thank W. Yang and N. Li (China Agricultural University) for providing the bacterial spot race T3 strain and for assistance with inoculation, and D. Bartlem (KWS) for critical reading and editing of an earlier draft of this manuscript. We also thank Y. Wang (Institute of Genetics and Developmental Biology, CAS) for help with Figure 1a. We thank L. Yan and Y. Li for assistance with tissue culture. This work was supported by grants from the National Key Research and Development Program of China (2016YFD0101804), the National Science Foundation of China (31788103 and 31420103912) and the Chinese Academy of Sciences (QYZDY-SSW-SMC030 and GJHZ1602) to C.G., and the Thousand Talents Plan to C.X.
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C.X. and C.G. designed the experiments; T.L., X.Y., Y.Y. and X.S. performed most of the experiments; X.Z. and W.D. generated transgenic plants. T.L., X.Y., Y.Y., X.S. and H.Z. analyzed the results; C.X. and C.G. supervised the project; C.X. and C.G. wrote the manuscript.
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Supplementary information
Supplementary Text and Figures
Supplementary Figures 1–8 (PDF 2568 kb)
Supplementary Sequences
The sequences of SP, SP5G, SlCLV3, SlWUS and SlGGP1 in S. pimpinellifolium (LA1589) (PDF 517 kb)
Supplementary Tables
Supplementary Tables 1–8 (PDF 1312 kb)
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Li, T., Yang, X., Yu, Y. et al. Domestication of wild tomato is accelerated by genome editing. Nat Biotechnol 36, 1160–1163 (2018). https://doi.org/10.1038/nbt.4273
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DOI: https://doi.org/10.1038/nbt.4273
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