Supplementary Figure 1: Molecular confirmation of the correct integration of the HDR-mediated event to generate dsxF | Nature Biotechnology

Supplementary Figure 1: Molecular confirmation of the correct integration of the HDR-mediated event to generate dsxF

From: A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes

Supplementary Figure 1

PCRs were performed to verify the location of the dsx ϕC31 knock-in integration. Primers (blue arrows) were designed to bind internal of the ϕC31 construct and outside of the regions used for homology directed repair (HDR) (dotted grey lines) which were included in the Donor plasmid K101. Amplicons of the expected sizes should only be produced in the event of a correct HDR integration. The gel shows PCRs performed on the 5’ (left) and 3’ (right) of 3 individuals for the dsx ϕC31 knock-in line (dsxF) and wild type (wt) as a negative control.

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