Supplementary Figure 10: Modeling the effect of unforeseen additional fitness reduction encountered by heterozygous gene drive females | Nature Biotechnology

Supplementary Figure 10: Modeling the effect of unforeseen additional fitness reduction encountered by heterozygous gene drive females

From: A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes

Supplementary Figure 10

Time dynamics of drive allele frequency as predicted by the deterministic model, with different coloured lines representing additional percentage reductions from zero to 100% in the baseline fertility of females, mimicking an ecologically more realistic scenario in which there were more severe fitness effects associated with the gene drive than in the laboratory. The reduction in fitness is assumed to affect the overall reproductive success (i.e mating success, longevity, fertility etc.). The baseline fertility values relative to the wild type are those reported in Supplementary Table S4 (0.65 for females with transgenic mothers, and 0.217 for females with transgenic fathers), that describes these and all other parameters estimated from experiment. Fitness reductions of up to 40% are predicted to crash the population. The spread of the drive is computed using the deterministic model in Figure 5.

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