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Unexpected CRISPR on-target effects

Cas9 can induce extensive on-target damage, including large deletions, inversions, and insertions.

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Figure 1: Outcomes of Cas9-catalyzed DNA double-strand break repair in human cells.


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Correspondence to Jin-Soo Kim.

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Competing interests

J.-S.K. is a cofounder of and holds stock in ToolGen, Inc., a company focused on therapeutic genome editing.

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Lee, H., Kim, JS. Unexpected CRISPR on-target effects. Nat Biotechnol 36, 703–704 (2018).

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