Supplementary Figure 7 : Overlap between unique PigA alleles derived using different methods.

From: Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

Supplementary Figure 7

“Single cell” and “PacBio” refer to alleles shown in Fig. S2B-D and Fig. 2A. “Plasmid” alleles were derived in the same experiment by Sanger sequencing of 3.5kb long PCR fragments cloned into plasmid vectors (primer pair 1F/1R, Fig. S2A). “RNP” alleles were derived by single cell cloning in an independent experiment only using guide #15 (Fig. S1A “Electroporation exp2”; Fig. S1B).