Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

A new route to synthetic DNA

An enzymatic approach to oligonucleotide synthesis promises to extend the length and fidelity of synthetic DNA

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Comparison of traditional phosphoramidite and enzymatic synthesis of oligos.

References

  1. Michelson, A.M. & Todd, A.R. J. Chem. Soc. 2632 (1955).

  2. McBride, L.J. & Caruthers, M.H. Tetrahedr. Lett. 24, 245–248 (1983).

    Article  CAS  Google Scholar 

  3. Palluk, S. et al. Nat. Biotechnol. 36, 645–650 (2018).

    Article  CAS  Google Scholar 

  4. Yoshimi, K., Kaneko, T., Voigt, B. & Mashimo, T. Nat. Commun. 5, 4240 (2014).

    Article  CAS  Google Scholar 

  5. Huertas, P. Nat. Struct. Mol. Biol. 17, 11–16 (2010).

    Article  CAS  Google Scholar 

  6. Goldman, N. et al. Nature 494, 77–80 (2013).

    Article  CAS  Google Scholar 

  7. Caruthers, M.H. Science 230, 281–285 (1985).

    Article  CAS  Google Scholar 

  8. Minhaz Ud-Dean, S.M. Syst. Synth. Biol. 2, 67–73 (2008).

    Article  CAS  Google Scholar 

  9. Efcavitch, J.W. & Siddiqi, S. US patent no. 8,808,989 (2014).

  10. Ybert, T. & Gariel, S. International application no. PCT/FR2015/052310 (2015).

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Adam Clore.

Ethics declarations

Competing interests

A.C. is employed by Integrated DNA Technologies, from which oligos use in the experiments described in Palluk et al. were purchased.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Clore, A. A new route to synthetic DNA. Nat Biotechnol 36, 593–595 (2018). https://doi.org/10.1038/nbt.4185

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt.4185

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing