Profiling DNA–transcription factor interactions

Global transcription factor activity is measured by screening cell extracts with an oligonucleotide library.

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Figure 1: ATI combines affinity-based enrichment of oligonucleotides bound by nuclear proteins isolated from cells or tissues with mass spectrometric measurements of protein abundance to determine actively engaging transcription factors.

References

  1. 1

    Wei, B. et al. Nat. Biotechnol. 36, 521–529 (2018).

    CAS  Article  Google Scholar 

  2. 2

    Oliphant, A.R., Brandl, C.J. & Struhl, K. Mol. Cell. Biol. 9, 2944–2949 (1989).

    CAS  Article  Google Scholar 

  3. 3

    Stoltenburg, R., Reinemann, C. & Strehlitz, B. Biomol. Eng. 24, 381–403 (2007).

    CAS  Article  Google Scholar 

  4. 4

    He, C. et al. Mol. Cell 64, 416–430 (2016).

    CAS  Article  Google Scholar 

  5. 5

    Zentner, G.E. & Henikoff, S. Nat. Rev. Genet. 15, 814–827 (2014).

    CAS  Article  Google Scholar 

  6. 6

    Skene, P.J. & Henikoff, S. eLife 6, e21856 (2017).

    Article  Google Scholar 

  7. 7

    Buenrostro, J.D., Wu, B., Chang, H.Y. & Greenleaf, W.J. Curr. Protoc. Mol. Biol. 109, 21.29.1–21.29.9 (2015).

    Article  Google Scholar 

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Correspondence to Marius Wernig.

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Ang, C., Wernig, M. Profiling DNA–transcription factor interactions. Nat Biotechnol 36, 501–502 (2018). https://doi.org/10.1038/nbt.4161

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