Profiling DNA–transcription factor interactions

Global transcription factor activity is measured by screening cell extracts with an oligonucleotide library.

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Figure 1: ATI combines affinity-based enrichment of oligonucleotides bound by nuclear proteins isolated from cells or tissues with mass spectrometric measurements of protein abundance to determine actively engaging transcription factors.


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Correspondence to Marius Wernig.

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The authors declare no competing financial interests.

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Ang, C., Wernig, M. Profiling DNA–transcription factor interactions. Nat Biotechnol 36, 501–502 (2018).

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