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Profiling DNA–transcription factor interactions

Nature Biotechnology volume 36, pages 501–502 (2018)Cite this article

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Global transcription factor activity is measured by screening cell extracts with an oligonucleotide library.

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Figure 1: ATI combines affinity-based enrichment of oligonucleotides bound by nuclear proteins isolated from cells or tissues with mass spectrometric measurements of protein abundance to determine actively engaging transcription factors.

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Authors and Affiliations

  1. Cheen Euong Ang and Marius Wernig are at the Institute for Stem Cell Biology and Regenerative Medicine and Department of Pathology, Stanford University, Stanford, California, USA,

    Cheen Euong Ang

  2. Cheen Euong Ang is at the Department of Bioengineering, Stanford University, Stanford, California, USA,

    Cheen Euong Ang & Marius Wernig

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  1. Cheen Euong Ang
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  2. Marius Wernig
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Correspondence to Marius Wernig.

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The authors declare no competing financial interests.

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Ang, C., Wernig, M. Profiling DNA–transcription factor interactions. Nat Biotechnol 36, 501–502 (2018). https://doi.org/10.1038/nbt.4161

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