Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Profiling DNA–transcription factor interactions

Global transcription factor activity is measured by screening cell extracts with an oligonucleotide library.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: ATI combines affinity-based enrichment of oligonucleotides bound by nuclear proteins isolated from cells or tissues with mass spectrometric measurements of protein abundance to determine actively engaging transcription factors.


  1. 1

    Wei, B. et al. Nat. Biotechnol. 36, 521–529 (2018).

    CAS  Article  Google Scholar 

  2. 2

    Oliphant, A.R., Brandl, C.J. & Struhl, K. Mol. Cell. Biol. 9, 2944–2949 (1989).

    CAS  Article  Google Scholar 

  3. 3

    Stoltenburg, R., Reinemann, C. & Strehlitz, B. Biomol. Eng. 24, 381–403 (2007).

    CAS  Article  Google Scholar 

  4. 4

    He, C. et al. Mol. Cell 64, 416–430 (2016).

    CAS  Article  Google Scholar 

  5. 5

    Zentner, G.E. & Henikoff, S. Nat. Rev. Genet. 15, 814–827 (2014).

    CAS  Article  Google Scholar 

  6. 6

    Skene, P.J. & Henikoff, S. eLife 6, e21856 (2017).

    Article  Google Scholar 

  7. 7

    Buenrostro, J.D., Wu, B., Chang, H.Y. & Greenleaf, W.J. Curr. Protoc. Mol. Biol. 109, 21.29.1–21.29.9 (2015).

    Article  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Marius Wernig.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Ang, C., Wernig, M. Profiling DNA–transcription factor interactions. Nat Biotechnol 36, 501–502 (2018).

Download citation


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing