Supplementary Figure 11: Behavioral examination of spatial learning abilities in organoid-grafted mice | Nature Biotechnology

Supplementary Figure 11: Behavioral examination of spatial learning abilities in organoid-grafted mice

From: An in vivo model of functional and vascularized human brain organoids

Supplementary Figure 11

(a) Latency to locate target during Barnes maze training of 3 trials per day for 3 days. Values for individual mice were averaged within each day for analysis. Training performance was analyzed by two-way ANOVA with group as a between subject factor and day as a within-subject factor. There was a significant effect of day (F(2,24) = 24.3, p < 0.0001), but no effect of group (F(1,12) = 0.8116, p = 0.3854) or group X day interaction (F(2,24) = 2.878, p = 0.0758). (b) Latency to locate target during Barnes maze probe trial on day 4, 24 hr after final training day. Probe trial performance was analyzed by the Mann-Whitney test. Unengrafted vs. grafted p = 0.1474. (c) Errors prior to locating target during Barnes maze training of 3 trials per day for 3 days. Values for individual mice were averaged within each day for analysis. There was a significant effect of day (F(2,24) = 7.244, p = 0.0035) and a group X day interaction (F(2,24) = 7.878, p = 0.0023; **post-hoc Sidak’s multiple comparison test p = 0.0052 for training Day 1, p = 0.7258 for Day 2, p = 0.9438 for Day 3). No main effect was observed for group (F(1,12) = 0.8008, p = 0.3884). (d) Pokes to target location during Barnes maze probe trial on day 4, 24 hr after final training day. **Unengrafted vs. grafted p = 0.0076 (e) Location of errors during probe trial for Nod-Scid mice. (f) Location of errors during probe trial for organoid implanted mice. Values in a-f represent mean ± s.e.m. for each group, n=7 mice per group.

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