Coronal sections (40μm) from anterior to posterior were mounted with DAPI, imaged by fluorescence microscopy and stitched post acquisition. A. hSyn1-SORTCAU injected into the striatum. B. hSyn1-SORTCAU injected into the dorsal hippocampus. C. GFAP-SORTCAU injected into the dorsal hippocampus. D., E. hSyn1-FLEX-SORTCAU injected into the dorsal hippocampus in Vgat-cre and Vglut-Cre mice, respectively. Scale bar 1000μm. Three animals were injected; two were used for SORT-M while one was used for fluorescence microscopy.