Supplementary Figure 5: SORT-M labeling of tagged proteins from rat dissociated neuron-glia co-cultures | Nature Biotechnology

Supplementary Figure 5: SORT-M labeling of tagged proteins from rat dissociated neuron-glia co-cultures

From: Labeling and identifying cell-specific proteomes in the mouse brain

Supplementary Figure 5

Lysates were prepared from dissociated neuron-glia co-cultures that had been transduced with the hSyn1-SORTCAU AAV in media supplemented with AlkK. Tagged proteins in the lysates were labelled with Az-AF647, analysed by SDS-PAGE and imaged for fluorescence. This experiment was independently repeated twice with similar results.

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