Supplementary Figure 10: Spatially controlled cell-type specific labeling in either neurons or glia. | Nature Biotechnology

Supplementary Figure 10: Spatially controlled cell-type specific labeling in either neurons or glia.

From: Labeling and identifying cell-specific proteomes in the mouse brain

Supplementary Figure 10

A. Coronal section from a wild-type mouse brain unilaterally injected with hSyn1-SORTCAU AAV in the hippocampal right hemisphere. Red, mCherry fluorescence; Blue: DAPI stain. Scale bar is 1000 μm. B. Neurons in the right hemisphere are specifically targeted for SORT. mCherry fluorescence and anti-NeuN and anti-GFAP immunostaining for right Striatum for a mouse unilaterally injected with hSyn1-SORTCAU AAV into the right hemisphere. The arrows point to examples of mCherry positive cells. Scale bar is 50 μm. C. SDSPAGE analysis of protein extracts fluorescently labeled with Az-AF647. Extracts are from the right (R) and left (L) hippocampus of mice unilaterally injected with hSyn1-SORTCAU into the right (R) hippocampus and provided with AlkK. D., E., F., As for A.,B.,C. but using GFAP-SORTCAU AAV. The injections were done in three animals, with two animals being used for SORT-M and one animal being used for immunohistochemistry and subsequent fluorescence and confocal microscopy.

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