Supplementary Figure 8: Comparing striatal SORT tagging efficiency to E.Coli SORT-tagging | Nature Biotechnology

Supplementary Figure 8: Comparing striatal SORT tagging efficiency to E.Coli SORT-tagging

From: Labeling and identifying cell-specific proteomes in the mouse brain

Supplementary Figure 8

A. E.Coli were SORTCAU tagged with AlkK, as previously described2 and labeled with Az-A647. The SORT-M labeled E. coli proteome was serially diluted with unlabelled proteome to decrease the percentage of labeling from 100 %. Equal amounts of each of the resulting E. coli proteomes and the striatal proteomes were loaded. The striatal protein lysate is from a mouse unilateral injected with hSyn1-SORTCAU into the right hemisphere. We estimate from densitometry that SORT labeling in striatum corresponds to about 40% of the maximal E. coli labeling. B. Coomassie stain of the same gel as load control. The experiment was performed once.

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