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DNA sequencing at ultra-high fidelity

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Built-in error-correction and novel fluorogenic chemistry open a path to perfect read quality.

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Figure 1: Workflow of ECC sequencing.

References

  1. Chen, Z. et al. Nat. Biotechnol. http://dx.doi.org/10.1038/nbt.3982 (2017).

  2. Chen, Z. et al. ChemBioChem 16, 1153–1157 (2015).

    Article  CAS  Google Scholar 

  3. Sims, P.A., Greenleaf, W.J., Duan, H. & Xie, X.S. Nat. Methods 8, 575–580 (2011).

    Article  CAS  Google Scholar 

  4. Rothberg, J.M. & Leamon, J.H. Nat. Biotechnol. 26, 1117–1124 (2008).

    Article  CAS  Google Scholar 

  5. Goodwin, S., McPherson, J.D. & McCombie, W.R. Nat. Rev. Genet. 17, 333–351 (2016).

    Article  CAS  Google Scholar 

  6. Usdin, K. Genome Res. 18, 1011–1019 (2008).

    Article  CAS  Google Scholar 

  7. Helisalmi, S. et al. Neurodegener. Dis. 14, 204–208 (2014).

    Article  CAS  Google Scholar 

  8. Eid, J. et al. Science 323, 133–138 (2009).

    Article  CAS  Google Scholar 

  9. Chin, C.S. et al. Nat. Methods 13, 1050–1054 (2016).

    Article  CAS  Google Scholar 

Download references

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Correspondence to Robert Sebra.

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The author declares no competing financial interests.

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Sebra, R. DNA sequencing at ultra-high fidelity. Nat Biotechnol 35, 1143–1144 (2017). https://doi.org/10.1038/nbt.4001

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