Table 1 Genome reporting standards for SAGs and MAGs

From: Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Criterion Description
Finished (SAG/MAG)
Assembly qualitya Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better
High-quality draft (SAG/MAG)
Assembly qualitya Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs.
Completionb >90%
Contaminationc <5%
Medium-quality draft (SAG/MAG)
Assembly qualitya Many fragments with little to no review of assembly other than reporting of standard assembly statistics.
Completionb ≥50%
Contaminationc <10%
Low-quality draft (SAG/MAG)
Assembly qualitya Many fragments with little to no review of assembly other than reporting of standard assembly statistics.
Completionb <50%
Contaminationc <10%
This is a compressed set of genome reporting standards for SAGs and MAGs. For a complete list of mandatory and optional standards, see Supplementary Table 1.
  1. aAssembly statistics include but are not limited to: N50, L50, largest contig, number of contigs, assembly size, percentage of reads that map back to the assembly, and number of predicted genes per genome.
  2. bCompletion: ratio of observed single-copy marker genes to total single-copy marker genes in chosen marker gene set.
  3. cContamination: ratio of observed single-copy marker genes in ≥2 copies to total single-copy marker genes in chosen marker gene set.