Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Single-minded CRISPR screening

Nature Biotechnology volume 35, pages 339–340 (2017)Cite this article

Subjects

Transcriptome-wide effects of gene knockouts are screened at the single-cell level by combining high-throughput, single-cell RNA-seq with lentiviral CRISPR libraries.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Pooled CRISPR screening strategies.

Marina Corral Spence / Springer Nature

References

  1. Wang, T., Wei, J.J., Sabatini, D.M. & Lander, E.S. Science 343, 80–84 (2014).

    Article  CAS  Google Scholar 

  2. Shalem, O. et al. Science 343, 84–87 (2014).

    Article  CAS  Google Scholar 

  3. Adamson, B. et al. Cell 167, 1867–1882.e21 (2016).

    Article  CAS  Google Scholar 

  4. Dixit, A. et al. Cell 167, 1853–1866.e17 (2016).

    Article  CAS  Google Scholar 

  5. Jaitin, D.A. et al. Cell 167, 1883–1896.e15 (2016).

    Article  CAS  Google Scholar 

  6. Datlinger, P. et al. Nat. Methods 14, 297–301 (2017).

    Article  CAS  Google Scholar 

  7. Junker, J.P. & van Oudenaarden, A. Cell 157, 8–11 (2014).

    Article  CAS  Google Scholar 

  8. Zetsche, B. et al. Nat. Biotechnol. 35, 31–34 (2017).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA

    Bryan R Lanning & Christopher R Vakoc

Authors
  1. Bryan R Lanning
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christopher R Vakoc
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Christopher R Vakoc.

Ethics declarations

Competing interests

Christopher Vakoc is an advisor to KSQ therapeutics.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lanning, B., Vakoc, C. Single-minded CRISPR screening. Nat Biotechnol 35, 339–340 (2017). https://doi.org/10.1038/nbt.3849

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt.3849

Search

Advanced search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research