Supplementary Figure 3 : Expression of fluorescently labeled harmonin-a1 and harmonin-b1 at 6 weeks in c.216AA organ of Corti after P1 dual vector injection.

From: Gene therapy restores auditory and vestibular function in a mouse model of Usher syndrome type 1c

Supplementary Figure 3

(a-c) Confocal images of the basal turn in 6 weeks old c.216AA mice after P1 co-injection of AAV2/Anc80L65.CMV.tdTomato::harmonin-a1 (0.5 μl; 4.1 × 1012 gc/ml) and AAV2/Anc80L65.CMV.EGFP::harmonin-b1 (0.5 μl; 3.0 × 1012 gc/ml). 69% and 74% of the total number of cells (IHCs and OHCs) expressed EGFP (a) and tdTomato (c), respectively and 65% expressed both markers demonstrating successful co-transduction. Total hair cells were counted based on the phalloidin image (b, blue). EGFP+ (a) and tdTomato+ (c) hair cells were counted based on the confocal images shown. Scale bar: 20 μm.