Supplementary Figure 5: Agreement of ΔE with bacterial fitness depends on strength of antibiotic selection | Nature Biotechnology

Supplementary Figure 5: Agreement of ΔE with bacterial fitness depends on strength of antibiotic selection

From: Mutation effects predicted from sequence co-variation

Supplementary Figure 5

(a) Many mutations to TEM-1 β-lactamase which are deleterious according to ΔE are only revealed as deleterious in vivo by increasing selective pressure through higher ampicillin concentrations (mutational sensitivity per position (average ΔE); left to right, shades of blue additionally indicate concentration of first significant effect determined by fitting a two-component Gaussian mixture model at 2500 μg/ml). (b) Agreement between ΔE and experimental effects for kanamycin kinase decreases as the concentration of antibiotic is increased to a point where most variants are completely depleted and intermediate fitness effects cannot be resolved anymore (mixture model fitted at 1:8 WT MIC in log space).

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