Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Bypassing GMO regulations with CRISPR gene editing

Nature Biotechnology volume 34, pages 1014–1015 (2016)Cite this article

Subjects

To the Editor:

The US Department of Agriculture (USDA) recently announced that regulation of a CRISPR-Cas9 gene-edited mushroom, in which a polyphenol oxidase (PPO) gene had been mutated to avoid (or delay) browning, fell outside of genetically modified organism (GMO) legislation1. However, we remain concerned that the approved mushroom may still contain tiny fragments of foreign DNA in its genome. If foreign DNA is present after CRISPR-Cas9 editing, regulatory approval would be required under current GMO legislation.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Insertions of Cas9 and gRNA plasmid-derived DNA sequences at the genomic target site in transiently transfected Arabidopsis protoplasts.

References

  1. Waltz, E. Nature 532, 293 (2016).

    Article  CAS  Google Scholar 

  2. Kim, S., Kim, D., Cho, S.W., Kim, J. & Kim, J.S. Genome Res. 24, 1012–1019 (2014).

    Article  CAS  Google Scholar 

  3. Kim, D. et al. Nat. Methods 12, 237–243 (2015).

    Article  CAS  Google Scholar 

  4. Woo, J.W. et al. Nat. Biotechnol. 33, 1162–1164 (2015).

    Article  CAS  Google Scholar 

  5. Kim, D. et al. Nat. Biotechnol. 34, 863–868 (2016).

    Article  CAS  Google Scholar 

  6. Hur, J.K. et al. Nat. Biotechnol. 34, 807–808 (2016).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by a grant from the Institute for Basic Science (IBS-R021-D1).

Author information

Authors and Affiliations

  1. Department of Chemistry, Seoul National University, Seoul, South Korea

    Jungeun Kim & Jin-Soo Kim

  2. Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea

    Jin-Soo Kim

Authors
  1. Jungeun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jin-Soo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

J.-S.K. wrote the manuscript. J.K. performed the plant experiment and analyzed the data.

Corresponding author

Correspondence to Jin-Soo Kim.

Ethics declarations

Competing interests

The authors have filed patent applications on Cas9 and Cpf1 RNP methods.

Supplementary information

Supplementary Methods

Supplementary methods (PDF 56 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, J., Kim, JS. Bypassing GMO regulations with CRISPR gene editing. Nat Biotechnol 34, 1014–1015 (2016). https://doi.org/10.1038/nbt.3680

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt.3680

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing