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Bypassing GMO regulations with CRISPR gene editing

Nature Biotechnology volume 34, pages 1014–1015 (2016)Cite this article

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To the Editor:

The US Department of Agriculture (USDA) recently announced that regulation of a CRISPR-Cas9 gene-edited mushroom, in which a polyphenol oxidase (PPO) gene had been mutated to avoid (or delay) browning, fell outside of genetically modified organism (GMO) legislation1. However, we remain concerned that the approved mushroom may still contain tiny fragments of foreign DNA in its genome. If foreign DNA is present after CRISPR-Cas9 editing, regulatory approval would be required under current GMO legislation.

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Figure 1: Insertions of Cas9 and gRNA plasmid-derived DNA sequences at the genomic target site in transiently transfected Arabidopsis protoplasts.

References

  1. Waltz, E. Nature 532, 293 (2016).

    Article  CAS  Google Scholar 

  2. Kim, S., Kim, D., Cho, S.W., Kim, J. & Kim, J.S. Genome Res. 24, 1012–1019 (2014).

    Article  CAS  Google Scholar 

  3. Kim, D. et al. Nat. Methods 12, 237–243 (2015).

    Article  CAS  Google Scholar 

  4. Woo, J.W. et al. Nat. Biotechnol. 33, 1162–1164 (2015).

    Article  CAS  Google Scholar 

  5. Kim, D. et al. Nat. Biotechnol. 34, 863–868 (2016).

    Article  CAS  Google Scholar 

  6. Hur, J.K. et al. Nat. Biotechnol. 34, 807–808 (2016).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by a grant from the Institute for Basic Science (IBS-R021-D1).

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Authors and Affiliations

  1. Department of Chemistry, Seoul National University, Seoul, South Korea

    Jungeun Kim & Jin-Soo Kim

  2. Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea

    Jin-Soo Kim

Authors
  1. Jungeun Kim
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  2. Jin-Soo Kim
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Contributions

J.-S.K. wrote the manuscript. J.K. performed the plant experiment and analyzed the data.

Corresponding author

Correspondence to Jin-Soo Kim.

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Competing interests

The authors have filed patent applications on Cas9 and Cpf1 RNP methods.

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Kim, J., Kim, JS. Bypassing GMO regulations with CRISPR gene editing. Nat Biotechnol 34, 1014–1015 (2016). https://doi.org/10.1038/nbt.3680

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