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Saturation analysis for whole-genome bisulfite sequencing data

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Figure 1: Single-replicate analysis.
Figure 2: Saturation analysis of deep replicate methylomes.
Figure 3: RRBS spike-in simulation.

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Acknowledgements

We thank the National Institute for Health Research (NIHR) Cambridge BioResource volunteers for participation, staff for volunteer recruitment, S.A.B. and Management Committee for support and the NIHR Cambridge Biomedical Research Centre for funding. S.C.H., M.G., I.G.G. and H.G.S. were supported by EU-FP7 project BLUEPRINT (282510). M.J.Z. and A.M. were supported by the US National Institutes of Health Common Fund (U01ES017155). M.F. was supported by the BHF Cambridge Centre of Excellence (RE/13/6/30180). W.H.O. was supported by EU-FP7 project BLUEPRINT (282510), the NIHR, the British Heart Foundation (RP-PG-0310-1002, RG/09/12/28096) and the NHS Blood and Transplant. J.H. was supported by The Monument Trust. E.L. and S.B. were supported by EU-FP7 projects EpiTrain (316758), EpiGeneSys (257082) and BLUEPRINT (282510), the Wellcome Trust (99148) and a Royal Society Wolfson Research Merit Award (WM100023).

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Correspondence to Emanuele Libertini or Stephan Beck.

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A.C. and V.R. are employees of Illumina Inc., a public company that develops and markets systems for genetic analysis.

Supplementary information

Supplementary Methods and Table 1

Summary of methylomes included in the analysis. (PDF 64 kb)

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Libertini, E., Heath, S., Hamoudi, R. et al. Saturation analysis for whole-genome bisulfite sequencing data. Nat Biotechnol 34, 691–693 (2016). https://doi.org/10.1038/nbt.3524

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