Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Cas9-triggered chain ablation of cas9 as a gene drive brake

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: Schematics and proof of principle of CATCHA.


  1. Hsu, P.D., Lander, E.S. & Zhang, F. Cell 157, 1262–1278 (2014).

    CAS  Article  Google Scholar 

  2. Doudna, J.A. & Charpentier, E. Science 346, 1258096 (2014).

    Article  Google Scholar 

  3. DiCarlo, J.E. et al. bioRxiv (2015). doi:10.1101/013896

  4. Gantz, V.M. & Bier, E. Science 348, 442–444 (2015).

    CAS  Article  Google Scholar 

  5. Esvelt, K.M., Smidler, A.L., Catteruccia, F. & Church, G.M. Elife doi:10.7554/eLife.03401 (17 July 2014).

  6. Akbari, O.S. et al. Science 349, 927–929 (2015).

    CAS  Article  Google Scholar 

  7. Gratz, S.J. et al. Genetics 196, 961–971 (2014).

    CAS  Article  Google Scholar 

  8. Hay, B., Jan, L.Y. & Jan, Y.N. Cell 55, 577–587 (1988).

    CAS  Article  Google Scholar 

  9. Lasko, P.F. & Ashburner, M. Genes Dev. 4, 905–921 (1990).

    CAS  Article  Google Scholar 

  10. Port, F., Chin, H.-M., Lee, T. & Bullock, S.L. Proc. Natl. Acad. Sci. USA 111, E2967–E2976 (2014).

    CAS  Article  Google Scholar 

Download references


We thank D. Luginbuhl for generating transgenic flies, F. Port for providing gRNA flies, and J. Luo, T. Mosca, X. Wang, J. Li and J. Lui for advice and critical comments. This study was supported by an NIH grant (R01-DC005982) to L.L. X.J.G. was supported by an Enlight Foundation Interdisciplinary Fellowship. L.L. is an HHMI investigator.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Liqun Luo.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Integrated supplementary information

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1 and 2, Supplementary Table 1 and Supplementary Methods (PDF 1170 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Wu, B., Luo, L. & Gao, X. Cas9-triggered chain ablation of cas9 as a gene drive brake. Nat Biotechnol 34, 137–138 (2016).

Download citation

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing