Supplementary Figure 2 : Reversal of drive-induced ADE2 loss by an overwriting drive.

From: Safeguarding CRISPR-Cas9 gene drives in yeast

Supplementary Figure 2

Haploid yeast containing a complete autonomous ADE2-disrupting gene drive were mated with haploids containing an overwriting drive that restores ADE2 function. 15 diploid offspring were sporulated, dissected, and plated on adenine-limited plates. The resulting cream-colored colonies indicate that an intact ADE2 gene is present in all progeny, indicative of the ADE2-restoring drive successfully cutting and replacing the ADE2-disrupting gene drive.