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RNA structure from deep sequencing

Nature Biotechnology volume 33, pages 928–929 (2015)Cite this article

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A method to identify interacting regions in a folded RNA is a step toward solving RNA structures from sequencing data.

Solving the three-dimensional structure of an RNA molecule means laborious study by X-ray crystallography or nuclear magnetic resonance spectroscopy. However, faster complementary methods are on the horizon, many involving deep sequencing and sophisticated computational analysis. In this issue, Ramani et al.1 use deep sequencing and proximity ligation to identify nucleotide regions that interact in folded RNA molecules. The method provides an entirely new source of information on intramolecular RNA interactions that, with further improvement, may enable accurate prediction of RNA structure.

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Figure 1: Development of RPL method.

References

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Authors and Affiliations

  1. Eric Westhof is at Architecture & Reactivity of RNA, University of Strasbourg, Institute of Molecular and Cellular Biology of the CNRS, Strasbourg, France.,

    Eric Westhof

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  1. Eric Westhof
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Correspondence to Eric Westhof.

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The author declares no competing financial interests.

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Westhof, E. RNA structure from deep sequencing. Nat Biotechnol 33, 928–929 (2015). https://doi.org/10.1038/nbt.3338

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  • DOI: https://doi.org/10.1038/nbt.3338

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