Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Commentary
  • Published:

The smart and gentle microscope

New instruments are needed to realize the potential of quantitative and systematic imaging of living samples. But what would such a microscope look like?

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2

References

  1. Hyman, A.A. Phil. Trans. R. Soc. Lond. B 366, 3635–3637 (2011).

    Article  CAS  Google Scholar 

  2. Keller, P.J. Science 340, 1234168 (2013).

    Article  Google Scholar 

  3. Hooke, R. Micrographia (J. Martyn and J. Allestry, London, 1665).

    Google Scholar 

  4. Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Nat. Rev. Mol. Cell Biol. 3, 906–918 (2002).

    Article  CAS  Google Scholar 

  5. Stelzer, E.H.K. Cell 159, 1243–1246 (2014).

    Article  CAS  Google Scholar 

  6. Megason, S.G. & Fraser, S.E. Cell 130, 784–795 (2007).

    Article  CAS  Google Scholar 

  7. Cole, R. Cell Adhes. Migr. 8, 452–459 (2014).

    Article  Google Scholar 

  8. Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H.K. Science 305, 1007–1009 (2004).

    Article  CAS  Google Scholar 

  9. Amat, F. et al. Nat. Methods 11, 951–958 (2014).

    Article  CAS  Google Scholar 

  10. Conrad, C. et al. Nat. Methods 8, 246–249 (2011).

    Article  CAS  Google Scholar 

  11. Wang, K. et al. Nat. Methods 11, 625–628 (2014).

    Article  CAS  Google Scholar 

  12. Sharpe, J. et al. Science 296, 541–545 (2002).

    Article  CAS  Google Scholar 

  13. Bassi, A., Schmid, B. & Huisken, J. Development 142, 1016–1020 (2015).

    Article  CAS  Google Scholar 

  14. Planchon, T.A. et al. Nat. Methods 8, 417–423 (2011).

    Article  CAS  Google Scholar 

  15. Peng, H. et al. Nat. Commun. 5, 4342 (2014).

    Article  CAS  Google Scholar 

  16. Schmid, B. et al. Nat. Commun. 4, 2207 (2013).

    Article  Google Scholar 

  17. Chaloner, K. & Verdinelli, I. Stat. Sci. 10, 273–304 (1995).

    Article  Google Scholar 

  18. Seeger, M.W. & Wipf, D.P. IEEE Signal Process. Mag. 27, 81–91 (2010).

    Article  Google Scholar 

  19. Hawrylycz, M.J. et al. Nature 489, 391–399 (2012).

    Article  CAS  Google Scholar 

  20. Kaufmann, A., Mickoleit, M., Weber, M. & Huisken, J. Development 139, 3242–3247 (2012).

    Article  CAS  Google Scholar 

  21. Fahrbach, F.O., Voigt, F.F., Schmid, B., Helmchen, F. & Huisken, J. Optics Express 21, 21010–21026 (2013).

    Article  Google Scholar 

  22. Bouchard, M.B. et al. Nat. Photonics 9, 113–119 (2015).

    Article  CAS  Google Scholar 

  23. Ji, N., Magee, J.C. & Betzig, E. Nat. Methods 5, 197–202 (2008).

    Article  CAS  Google Scholar 

  24. Verveer, P.J. et al. Nat. Methods 4, 311–313 (2007).

    Article  CAS  Google Scholar 

  25. Schmid, B. & Huisken, J. Bioinformatics, 10.1093/bioinformatics/btv387 (25 June 2015).

Download references

Acknowledgements

We thank R. Power and G. Shah for their thoughtful comments on this manuscript.

Author information

Authors and Affiliations

Authors

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Scherf, N., Huisken, J. The smart and gentle microscope. Nat Biotechnol 33, 815–818 (2015). https://doi.org/10.1038/nbt.3310

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt.3310

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing