New instruments are needed to realize the potential of quantitative and systematic imaging of living samples. But what would such a microscope look like?
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References
Hyman, A.A. Phil. Trans. R. Soc. Lond. B 366, 3635–3637 (2011).
Keller, P.J. Science 340, 1234168 (2013).
Hooke, R. Micrographia (J. Martyn and J. Allestry, London, 1665).
Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Nat. Rev. Mol. Cell Biol. 3, 906–918 (2002).
Stelzer, E.H.K. Cell 159, 1243–1246 (2014).
Megason, S.G. & Fraser, S.E. Cell 130, 784–795 (2007).
Cole, R. Cell Adhes. Migr. 8, 452–459 (2014).
Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H.K. Science 305, 1007–1009 (2004).
Amat, F. et al. Nat. Methods 11, 951–958 (2014).
Conrad, C. et al. Nat. Methods 8, 246–249 (2011).
Wang, K. et al. Nat. Methods 11, 625–628 (2014).
Sharpe, J. et al. Science 296, 541–545 (2002).
Bassi, A., Schmid, B. & Huisken, J. Development 142, 1016–1020 (2015).
Planchon, T.A. et al. Nat. Methods 8, 417–423 (2011).
Peng, H. et al. Nat. Commun. 5, 4342 (2014).
Schmid, B. et al. Nat. Commun. 4, 2207 (2013).
Chaloner, K. & Verdinelli, I. Stat. Sci. 10, 273–304 (1995).
Seeger, M.W. & Wipf, D.P. IEEE Signal Process. Mag. 27, 81–91 (2010).
Hawrylycz, M.J. et al. Nature 489, 391–399 (2012).
Kaufmann, A., Mickoleit, M., Weber, M. & Huisken, J. Development 139, 3242–3247 (2012).
Fahrbach, F.O., Voigt, F.F., Schmid, B., Helmchen, F. & Huisken, J. Optics Express 21, 21010–21026 (2013).
Bouchard, M.B. et al. Nat. Photonics 9, 113–119 (2015).
Ji, N., Magee, J.C. & Betzig, E. Nat. Methods 5, 197–202 (2008).
Verveer, P.J. et al. Nat. Methods 4, 311–313 (2007).
Schmid, B. & Huisken, J. Bioinformatics, 10.1093/bioinformatics/btv387 (25 June 2015).
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We thank R. Power and G. Shah for their thoughtful comments on this manuscript.
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Scherf, N., Huisken, J. The smart and gentle microscope. Nat Biotechnol 33, 815–818 (2015). https://doi.org/10.1038/nbt.3310
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DOI: https://doi.org/10.1038/nbt.3310
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