Abstract
The brown planthopper (BPH) is the most destructive pest of rice (Oryza sativa) and a substantial threat to rice production, causing losses of billions of dollars annually1,2. Breeding of resistant cultivars is currently hampered by the rapid breakdown of BPH resistance2. Thus, there is an urgent need to identify more effective BPH-resistance genes. Here, we report molecular cloning and characterization of Bph3, a locus in rice identified more than 30 years ago that confers resistance to BPH. We show that Bph3 is a cluster of three genes encoding plasma membrane–localized lectin receptor kinases (OsLecRK1-OsLecRK3). Introgression of Bph3 into susceptible rice varieties by transgenic or marker-assisted selection strategies significantly enhanced resistance to both the BPH and the white back planthopper. Our results suggest that these lectin receptor kinase genes function together to confer broad-spectrum and durable insect resistance and provide a resource for molecular breeding of insect-resistant rice cultivars.
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Acknowledgements
This work was supported by Jiangsu 333 Program (BRA2012126), NSFC (31471470), 863 Program of China (2012AA101101, 2011AA10A101 and 2011BAD35B02-02), National Science and Technology Supporting Program (2011BAD35B02-02), National Key Transformation Program (2014ZX08001-001), Jiangsu Science and Technology Development Program (BE2012303, BK2010016), and the Jiangsu PAPD program. We thank R. Guan (Nanjing Agricultural University) and J. Wang (Chinese Academy of Agricultural Sciences) for statistical analysis, and J. Zhou (Chinese Academy of Sciences), W. Terzaghi (Wilkes University) and C. Lin (UC-LA) for proofreading the manuscript.
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Y.Q.L., H.K., Z.S., G.P. and J.He performed genetic analysis and mapping of Bph3; X. Zhang, X.G. and L.J. performed the genetic transformation; H. Wu, H.C., Q.W. and F.Z. performed the construction of vectors; K.Z., Y.R. and Y.W. performed the subcellular localization; X. Zheng, Z.Z., Q.L. and F.W. performed sequence analysis; Y.L.L., J. Hu, L.C. and X.C. performed the phenotypic analysis. J.W., H. Wang and C.W. directed the project and wrote the manuscript.
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Liu, Y., Wu, H., Chen, H. et al. A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice. Nat Biotechnol 33, 301–305 (2015). https://doi.org/10.1038/nbt.3069
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DOI: https://doi.org/10.1038/nbt.3069
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