Letter | Published:

In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9

Nature Biotechnology volume 33, pages 102106 (2015) | Download Citation

Abstract

Probing gene function in the mammalian brain can be greatly assisted with methods to manipulate the genome of neurons in vivo. The clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease (Cas)9 from Streptococcus pyogenes (SpCas9)1 can be used to edit single or multiple genes in replicating eukaryotic cells, resulting in frame-shifting insertion/deletion (indel) mutations and subsequent protein depletion. Here, we delivered SpCas9 and guide RNAs using adeno-associated viral (AAV) vectors to target single (Mecp2) as well as multiple genes (Dnmt1, Dnmt3a and Dnmt3b) in the adult mouse brain in vivo. We characterized the effects of genome modifications in postmitotic neurons using biochemical, genetic, electrophysiological and behavioral readouts. Our results demonstrate that AAV-mediated SpCas9 genome editing can enable reverse genetic studies of gene function in the brain.

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Acknowledgements

We thank A. Trevino and C. Le for technical assistance and the entire Zhang lab for technical support and critical discussions; we thank R. Platt (Broad Institute) and H. Worman (Columbia University) for sharing plasmids, R. Rikhye for providing a template for electrophysiology analysis; and X. Yu for statistical discussions. L.S. is a European Molecular Biology Organization (EMBO) Fellow and is supported by the Foundation for Polish Science. M.H. is supported by the Human Frontiers Scientific Program. A.B. holds a postdoctoral fellowship from the Simons Center for the Social Brain. N.H. is an EMBO Fellow and Y.L. is supported by Friends of the McGovern Institute Fellowship. M.S. is supported by grants from the US National Institutes of Health (NIH) (R01EY007023 and R01MH085802) and the Simons Foundation. F.Z. is supported by the National Institute of Mental Health (NIMH) through NIH Director's Pioneer Award (5DP1-MH100706), the NINDS through a NIH Transformative R01 grant (5R01-NS073124), the Keck, Merkin, Vallee, Damon Runyon, Searle Scholars, Klarman Family Foundation, Klingenstein, Poitras and Simons Foundations, and Bob Metcalfe. The authors plan on making the reagents widely available to the academic community through Addgene and to provide software tools via the Zhang lab website (http://www.genome-engineering.org/).

Author information

Author notes

    • Lukasz Swiech
    •  & Matthias Heidenreich

    These authors contributed equally to this work.

Affiliations

  1. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

    • Lukasz Swiech
    • , Matthias Heidenreich
    • , Naomi Habib
    • , Yinqing Li
    • , John Trombetta
    •  & Feng Zhang
  2. McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Lukasz Swiech
    • , Matthias Heidenreich
    • , Naomi Habib
    • , Yinqing Li
    •  & Feng Zhang
  3. McGovern Institute for Brain Research, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Lukasz Swiech
    • , Matthias Heidenreich
    • , Naomi Habib
    •  & Feng Zhang
  4. Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Abhishek Banerjee
    •  & Mriganka Sur
  5. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Yinqing Li

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Contributions

L.S., M.H. and F.Z. developed the concept and designed experiments. L.S. and M.H. carried out CRISPR-Cas9-related experiments and analyzed data. A.B. designed and performed electrophysiological experiments and analyzed data. N.H., Y.L. and J.T. carried-out RNA sequencing experiments and analyzed data. Y.L. analyzed NGS data. L.S., M.H. and F.Z. wrote the manuscript with input from all authors.

Competing interests

F.Z. is a scientific advisor of Editas Medicine and Horizon Discovery. A patent application has been filed relating to this work.

Corresponding author

Correspondence to Feng Zhang.

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DOI

https://doi.org/10.1038/nbt.3055

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