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The devil in the details of RNA-seq

Large-scale consortium efforts provide a thorough understanding of RNA-seq.

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Figure 1: Combinations of input RNA, library preparation protocols and sequencing technology generate different read output.

References

  1. SEQC/MAQC-III Consortium. Nat. Biotech. doi:10.1038/nbt.2957 (24 August 2014).

  2. Li, S. et al. Nat. Biotech. doi:10.1038/nbt.3000 (24 August 2014).

  3. Wang, C. et al. Nat. Biotech. doi:10.1038/nbt.3001 (24 August 2014).

  4. Li, S. et al. Nat. Biotech. doi:10.1038/nbt.2972 (24 August 2014).

  5. ENCODE Project Consortium. Nature 489, 57–74 (2012).

  6. Forrest, A.R.R. et al. Nature 507, 462–470 (2014).

    Article  CAS  Google Scholar 

  7. Hoheisel, J.D. Nat. Rev. Microbiol. 7, 200–210 (2006).

    Article  CAS  Google Scholar 

  8. Faulkner, G.J. et al. Nat. Genet. 41, 563–571 (2009).

    Article  CAS  Google Scholar 

  9. Risso, D., Ngai, J., Speed, T.P. & Dudoit, S. Nat. Biotech. doi:10.1038/nbt.2931 (24 August 2014).

  10. Itoh, M. et al. PLoS ONE http://dx.doi.org/doi:10.1371/journal.pone.0030809 (30 January 2012).

  11. Rung, J. & Brazma, A. Nat. Rev. Genet. 14, 89–99 (2013).

    Article  CAS  Google Scholar 

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Correspondence to Anton Kratz or Piero Carninci.

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Kratz, A., Carninci, P. The devil in the details of RNA-seq. Nat Biotechnol 32, 882–884 (2014). https://doi.org/10.1038/nbt.3015

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