Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

The devil in the details of RNA-seq

Large-scale consortium efforts provide a thorough understanding of RNA-seq.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Combinations of input RNA, library preparation protocols and sequencing technology generate different read output.


  1. SEQC/MAQC-III Consortium. Nat. Biotech. doi:10.1038/nbt.2957 (24 August 2014).

  2. Li, S. et al. Nat. Biotech. doi:10.1038/nbt.3000 (24 August 2014).

  3. Wang, C. et al. Nat. Biotech. doi:10.1038/nbt.3001 (24 August 2014).

  4. Li, S. et al. Nat. Biotech. doi:10.1038/nbt.2972 (24 August 2014).

  5. ENCODE Project Consortium. Nature 489, 57–74 (2012).

  6. Forrest, A.R.R. et al. Nature 507, 462–470 (2014).

    Article  CAS  Google Scholar 

  7. Hoheisel, J.D. Nat. Rev. Microbiol. 7, 200–210 (2006).

    Article  CAS  Google Scholar 

  8. Faulkner, G.J. et al. Nat. Genet. 41, 563–571 (2009).

    Article  CAS  Google Scholar 

  9. Risso, D., Ngai, J., Speed, T.P. & Dudoit, S. Nat. Biotech. doi:10.1038/nbt.2931 (24 August 2014).

  10. Itoh, M. et al. PLoS ONE (30 January 2012).

  11. Rung, J. & Brazma, A. Nat. Rev. Genet. 14, 89–99 (2013).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding authors

Correspondence to Anton Kratz or Piero Carninci.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kratz, A., Carninci, P. The devil in the details of RNA-seq. Nat Biotechnol 32, 882–884 (2014).

Download citation

  • Published:

  • Issue Date:

  • DOI:


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing