Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Next-generation sequencing in the clinic

Pools of cell lines carrying a variety of known mutations are used to validate the performance of a cancer diagnostic test based on next-generation sequencing.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type



Prices may be subject to local taxes which are calculated during checkout

Figure 1: Clinical sequencing test protocol and validation.


  1. Frampton, G.M. et al. Nat. Biotechnol. 31, 1023–1031 (2013).

    Article  CAS  Google Scholar 

  2. Conti, R.M. et al. J. Clin. Oncol. 31, 1134–1139 (2013).

    Article  CAS  Google Scholar 

  3. Gargis, A.S. et al. Nat. Biotechnol. 30, 1033–1036 (2012).

    Article  CAS  Google Scholar 

  4. Rehm, H.L. et al. Genet. Med. 15, 733–747 (2013).

    Article  Google Scholar 

  5. CAP Laboratory Accreditation Program. Molecular Pathology Checklist (College of American Pathologists, 2013).

  6. Bose, R. et al. Cancer Discov. 3, 224–237 (2013).

    Article  CAS  Google Scholar 

  7. Krzyzanowska, M.K. J. Clin. Oncol. 31, 1125–1127 (2013).

    Article  CAS  Google Scholar 

  8. Alberts, S.R. et al. J. Am. Med. Assoc. 307, 1383–1393 (2012).

    Article  CAS  Google Scholar 

  9. Garber, K. J. Natl. Cancer Inst. 103, 84–86 (2011).

    Article  Google Scholar 

  10. Simon, R. & Roychowdhury, S. Nat. Rev. Drug Discov. 12, 358–369 (2013).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Jason Y Park.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Park, J., Kricka, L. & Fortina, P. Next-generation sequencing in the clinic. Nat Biotechnol 31, 990–992 (2013).

Download citation

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links

Nature Briefing: Cancer

Sign up for the Nature Briefing: Cancer newsletter — what matters in cancer research, free to your inbox weekly.

Get what matters in cancer research, free to your inbox weekly. Sign up for Nature Briefing: Cancer