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Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

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Figure 1: Generation of gene mutant rats with a CRISPR-Cas system.


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We thank F. Zhang of the Broad Institute of MIT and Harvard for kindly providing us with the Cas9 expression vector. We thank S. Siwko for comments and advice. We also thank S.S. Bae and J.-S. Kim of Seoul National University for helping us to predict the potential off-target sites. This work was partially supported by grants from the State Key Development Programs of China (2012CB910400 to M.L., 2010CB945403 to D.L.), grants from the National Natural Science Foundation of China (31171318 to D.L. and 30930055 to M.L.), a grant from the Science and Technology Commission of Shanghai Municipality (11DZ2260300) and grants from the Program for Changjiang Scholars and Innovative Research Team in University (IRT1119 and IRT1128).

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Correspondence to Dali Li, Yongxiang Zhao or Mingyao Liu.

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Supplementary Methods, Supplementary Figures 1–9 and Supplementary Table 1 (PDF 936 kb)

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Li, D., Qiu, Z., Shao, Y. et al. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol 31, 681–683 (2013).

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