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Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

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Figure 1: Targeted mutagenesis in planta using the Cas9 RNA-guided endonuclease.
Figure 2: Measurement of the mutation rate induced by Cas9 RNA-guided endonuclease and transgenic N. benthamiana plants carrying mutations in the PDS gene.


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We thank S. Marillonnet and Icon Genetics, Halle, Germany for providing plasmid vectors, J. Win and S. Dong for help with figure preparation, and M. Smoker for help with the plant transformation. This work was supported by the Gatsby Charitable Foundation, the European Research Council (ERC), and the Biotechnology and Biological Sciences Research Council (BBSRC).

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Correspondence to Jonathan D G Jones or Sophien Kamoun.

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V.N., J.D.G.J. and S.K. have filed a patent application based on this work.

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Supplementary Material and Methods, Supplementary Figures 1–8 and Supplementary Tables 1–3 (PDF 3277 kb)

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Nekrasov, V., Staskawicz, B., Weigel, D. et al. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol 31, 691–693 (2013).

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