Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type



Prices may be subject to local taxes which are calculated during checkout

Figure 1: Targeted mutagenesis in planta using the Cas9 RNA-guided endonuclease.
Figure 2: Measurement of the mutation rate induced by Cas9 RNA-guided endonuclease and transgenic N. benthamiana plants carrying mutations in the PDS gene.


  1. Griggs, D. et al. Nature 495, 305–307 (2013).

    Article  CAS  Google Scholar 

  2. Voytas, D. Annu. Rev. Plant Biol. 64, 327–350 (2013).

    Article  CAS  Google Scholar 

  3. Mussolino, C. & Cathomen, T. Nat. Biotechnol. 31, 208–209 (2013).

    Article  CAS  Google Scholar 

  4. Goodin, M. et al. Mol. Plant Microbe Interact. 21, 1015–1026 (2008).

    Article  CAS  Google Scholar 

  5. Mali, P. et al. Science 339, 823–826 (2013).

    Article  CAS  Google Scholar 

  6. Van der Hoorn, R. Mol. Plant Microbe Interact. 13, 439–446 (2000).

    Article  CAS  Google Scholar 

  7. Cong, L. et al. Science 339, 819–823 (2013).

    Article  CAS  Google Scholar 

  8. Qi, Y. et al. Genome Res. 23, 547–554 (2013).

    Article  CAS  Google Scholar 

  9. Fu, Y. et al. Nat. Biotechnol. advance online publication, doi:10.1038/nbt.2623 (2013).

Download references


We thank S. Marillonnet and Icon Genetics, Halle, Germany for providing plasmid vectors, J. Win and S. Dong for help with figure preparation, and M. Smoker for help with the plant transformation. This work was supported by the Gatsby Charitable Foundation, the European Research Council (ERC), and the Biotechnology and Biological Sciences Research Council (BBSRC).

Author information

Authors and Affiliations


Corresponding authors

Correspondence to Jonathan D G Jones or Sophien Kamoun.

Ethics declarations

Competing interests

V.N., J.D.G.J. and S.K. have filed a patent application based on this work.

Supplementary information

Supplementary Text and Figures

Supplementary Material and Methods, Supplementary Figures 1–8 and Supplementary Tables 1–3 (PDF 3277 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nekrasov, V., Staskawicz, B., Weigel, D. et al. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol 31, 691–693 (2013).

Download citation

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing