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Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

Nature Biotechnology volume 31, pages 691–693 (2013)Cite this article

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Figure 1: Targeted mutagenesis in planta using the Cas9 RNA-guided endonuclease.
Figure 2: Measurement of the mutation rate induced by Cas9 RNA-guided endonuclease and transgenic N. benthamiana plants carrying mutations in the PDS gene.

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Acknowledgements

We thank S. Marillonnet and Icon Genetics, Halle, Germany for providing plasmid vectors, J. Win and S. Dong for help with figure preparation, and M. Smoker for help with the plant transformation. This work was supported by the Gatsby Charitable Foundation, the European Research Council (ERC), and the Biotechnology and Biological Sciences Research Council (BBSRC).

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Author notes

  1. Jonathan D G Jones and Sophien Kamoun: These authors contributed equally to this work.

Authors and Affiliations

  1. The Sainsbury Laboratory, Norwich Research Park, Norwich, UK

    Vladimir Nekrasov, Jonathan D G Jones & Sophien Kamoun

  2. Department of Plant and Microbial Biology, University of California, Berkeley, California, USA

    Brian Staskawicz

  3. Max Planck Institute for Developmental Biology, Tübingen, Germany

    Detlef Weigel

Authors
  1. Vladimir Nekrasov
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  2. Brian Staskawicz
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  3. Detlef Weigel
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  4. Jonathan D G Jones
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  5. Sophien Kamoun
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Corresponding authors

Correspondence to Jonathan D G Jones or Sophien Kamoun.

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Competing interests

V.N., J.D.G.J. and S.K. have filed a patent application based on this work.

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Supplementary Material and Methods, Supplementary Figures 1–8 and Supplementary Tables 1–3 (PDF 3277 kb)

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Nekrasov, V., Staskawicz, B., Weigel, D. et al. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol 31, 691–693 (2013). https://doi.org/10.1038/nbt.2655

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