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Targeted genome modification of crop plants using a CRISPR-Cas system

Nature Biotechnology volume 31, pages 686–688 (2013)Cite this article

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Figure 1: Genome editing in rice and wheat using an engineered type II CRISPR-Cas system.
Figure 2: HDR-mediated genome modification in rice protoplasts.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (201263, 383601 and 31200273), the Ministry of Agriculture of China (2011ZX08002-004 and 2013ZX08010-002) and Chinese Academy of Sciences (KSCX2-EW-N-06, KSCX2-EW-J-6). We thank X. Wang for bioinformatics analysis.

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Author notes

  1. Qiwei Shan, Yanpeng Wang and Jun Li: These authors contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

    Qiwei Shan, Yanpeng Wang, Jun Li, Yi Zhang, Kunling Chen, Zhen Liang, Kang Zhang, Jinxing Liu & Caixia Gao

  2. Institute of Molecular Medicine, Peking University, Beijing, China

    Jianzhong Jeff Xi

  3. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

    Jin-Long Qiu

Authors
  1. Qiwei Shan
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  2. Yanpeng Wang
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  3. Jun Li
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  4. Yi Zhang
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Corresponding authors

Correspondence to Jin-Long Qiu or Caixia Gao.

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Supplementary Methods, Supplementary Figures 1–5 and Supplementary Tables 1–7 (PDF 1175 kb)

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Shan, Q., Wang, Y., Li, J. et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol 31, 686–688 (2013). https://doi.org/10.1038/nbt.2650

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