Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Cracking the code of human T-cell immunity

Combinatorial tetramer staining coupled with mass cytometry allows simultaneous detection of T cells specific for a wide array of peptide epitopes.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: Overview of peptide-MHC tetramer multiplexing strategy with metal barcodes.


  1. Newell, E.W. et al. Nat. Biotechnol. 31, 623–629 (2013).

    Article  CAS  Google Scholar 

  2. Parks, D.R. & Herzenberg, L.A. Methods Enzymol. 108, 197–241 (1984).

    Article  CAS  Google Scholar 

  3. Chattopadhyay, P.K. & Roederer, M. Methods 57, 251–258 (2012).

    Article  CAS  Google Scholar 

  4. Bandura, D.R. et al. Anal. Chem. 81, 6813–6822 (2009).

    Article  CAS  Google Scholar 

  5. Bendall, S.C. et al. Science 332, 687–696 (2011).

    Article  CAS  Google Scholar 

  6. Newell, E.W., Sigal, N., Bendall, S.C., Nolan, G.P. & Davis, M.M. Immunity 36, 142–152 (2012).

    Article  CAS  Google Scholar 

  7. Altman, J.D. et al. Science 274, 94–96 (1996).

    Article  CAS  Google Scholar 

  8. Hadrup, S.R. et al. Nat. Methods 6, 520–526 (2009).

    Article  CAS  Google Scholar 

  9. Newell, E.W., Klein, L.O., Yu, W. & Davis, M.M. Nat. Methods 6, 497–499 (2009).

    Article  CAS  Google Scholar 

  10. Rodenko, B. et al. Nat. Protoc. 1, 1120–1132 (2006).

    Article  CAS  Google Scholar 

  11. Topalian, S.L. et al. N. Engl. J. Med. 366, 2443–2454 (2012).

    Article  CAS  Google Scholar 

  12. Hodi, F.S. et al. N. Engl. J. Med. 363, 711–723 (2010).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Kai W Wucherpfennig.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Harvey, C., Wucherpfennig, K. Cracking the code of human T-cell immunity. Nat Biotechnol 31, 609–610 (2013).

Download citation

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing