Brief Communication | Published:

TALEN-mediated editing of the mouse Y chromosome

Nature Biotechnology volume 31, pages 530532 (2013) | Download Citation

Abstract

The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes—Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.

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Acknowledgements

We thank K. Ganz and R. Flannery for support with animal care and experiments. We thank Jaenisch and Page laboratory members for helpful discussions on the manuscript. A.W.C. is supported by a Croucher scholarship. D.C.P. and Y.-C.H. were supported by Howard Hughes Medical Institute. R.J., H.W., S.M., G.G.W., C.S.S. and A.W.C. were supported by US National Institutes of Health grants R37-HD045022 and R01-CA084198. T.P. was supported by US National Institutes of Health grant R01-HG000257.

Author information

Author notes

    • Haoyi Wang
    •  & Yueh-Chiang Hu

    These authors contributed equally to this work.

Affiliations

  1. Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.

    • Haoyi Wang
    • , Yueh-Chiang Hu
    • , Styliani Markoulaki
    • , G Grant Welstead
    • , Albert W Cheng
    • , Chikdu S Shivalila
    • , Tatyana Pyntikova
    • , Daniel B Dadon
    • , David C Page
    •  & Rudolf Jaenisch
  2. Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Yueh-Chiang Hu
    •  & David C Page
  3. Computational and Systems Biology Program, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Albert W Cheng
  4. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

    • Chikdu S Shivalila
    • , Daniel B Dadon
    • , David C Page
    •  & Rudolf Jaenisch
  5. Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, Minnesota, USA.

    • Daniel F Voytas
  6. Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, New York, USA.

    • Adam J Bogdanove

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Contributions

H.W., Y.-C.H., D.C.P. and R.J. designed the experiments and wrote the manuscript. H.W., D.F.V. and A.J.B. designed TALENs. H.W. generated and tested TALENs. H.W. performed targeting experiments. C.S.S. and D.B.D. assisted with Southern blot analysis. S.M. performed tetraploid complementation experiments. Y.-C.H. and G.G.W. maintained mutant mouse colonies and performed animal-related experiments. T.P. performed FISH analysis. A.W.C. performed the off-target analysis. H.W., Y.-C.H. and G.G.W. analyzed the data.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to David C Page or Rudolf Jaenisch.

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    Supplementary Methods, Supplementary Figures 1–5 and Supplementary Tables 1–4

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DOI

https://doi.org/10.1038/nbt.2595

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