Figure 2: A.alpina homeotic flower and flowering-time mutants. | Nature Biotechnology

Figure 2: A.alpina homeotic flower and flowering-time mutants.

From: Mutation identification by direct comparison of whole-genome sequencing data from mutant and wild-type individuals using k-mers

Figure 2

(a) fde1 mutant flowers are deformed compared to flowers in wild-type plants. (b) pep1-1 mutant plants flower without vernalization compared to wild type, which has an obligate requirement for vernalization to flower. (c) Alignment of a NIKS contig of pep1-1 against the A. thaliana reference sequence reveals the splice-site mutation in the A. alpina ortholog of FLOWERING LOCUS C (FLC) previously reported to be responsible for the pep1-1 phenotype. The mutant base is shown in red, the exonic regions in A. thaliana are shown in yellow, the inferred exon of A. alpina in blue and all noncoding nucleotides in gray. (d) Alignment of a NIKS contig of fde1 against the A. thaliana reference sequence revealing a putative amino acid change likely to alter an aspartic acid to an asparagine within the 4th coding exon of the A. alpina ortholog of AP2. The frame used to translate the nucleotide sequence to an amino acid sequence was inferred from the annotation of A. thaliana. Causal mutations are shown in red. Exonic sequences associated with the mutation are shown in blue and exons of A. thaliana in yellow. Only relevant parts of the alignments are shown.

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