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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Nature Biotechnology volume 31, pages 230232 (2013) | Download Citation

Abstract

We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (2012-0001225) and ToolGen, Inc. We thank Jae Kyung Chon for bioinformatic analysis.

Author information

Author notes

    • Seung Woo Cho
    •  & Sojung Kim

    These authors contributed equally to this work.

Affiliations

  1. National Creative Research Initiatives Center for Genome Engineering, Seoul National University, Seoul, South Korea.

    • Seung Woo Cho
    • , Sojung Kim
    • , Jong Min Kim
    •  & Jin-Soo Kim
  2. Department of Chemistry, Seoul National University, Seoul, South Korea.

    • Seung Woo Cho
    • , Sojung Kim
    • , Jong Min Kim
    •  & Jin-Soo Kim

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Contributions

S.W.C., S.K. and J.M.K. performed the experiments. J.-S.K. wrote the manuscript.

Competing interests

S.W.C., S.K. and J.-S.K. have filed a patent based on this work.

Corresponding author

Correspondence to Jin-Soo Kim.

Supplementary information

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    Supplementary Text and Figures

    Supplementary Figures 1–6, Supplementary Methods and Supplementary Table 1

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DOI

https://doi.org/10.1038/nbt.2507

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