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Karyotypic abnormalities in human induced pluripotent stem cells and embryonic stem cells

Nature Biotechnology volume 29, pages 313–314 (2011)Cite this article

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Trisomy 12 is the predominant abnormality in both cell types, representing 42.6% of total aberrations detected in ESCs and 31.9% in iPSCs. Trisomy 8 occurs more frequently in iPSCs than in ESCs. Partial gain of chromosome 12 due to duplication of 12p or acquisition of an isochromosome 12p) and trisomy 20q (isochromosome 20q) are recurrent in both iPSC and ESC lines. An added X chromosome, recurrent in female ESC lines, occurs in one iPSC line. Notably, trisomy 17 is prevalent in ESC cultures but is never observed in this large data set of iPSC lines. Nonrecurrent abnormalities are common among the abnormalities found in this series: 39.3% of aberrations in ESCs and 42.0% in iPSCs. The karyotypes in this category are listed in Supplementary Table 2. Aberrations resulting in a gain of chromosome 1q sequences are frequent in ESCs, with an approximate region of overlap from 1q25 to 1q41, but a similar pattern is not observed in iPSCs.

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Figure 1: Recurrent chromosomal abnormalities in human ESC and iPSC cultures.

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  • 27 April 2011

    In the version of this article initially published, the first word of title was misspelled. The correct title is “Karyotypic abnormalities in human induced pluripotent stem cells and embryonic stem cells.” The error has been corrected in the HTML and PDF versions of the article.

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Acknowledgements

This project has been funded in part with federal funds from the National Center for Research Resources, National Institutes of Health, Department of Health and Human Services, under contract no. HHSN309200582085C.

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Authors and Affiliations

  1. WiCell Research Institute, Madison, Wisconsin, United States

    Seth M Taapken, Benjamin S Nisler, Tori L Sampsell-Barron, Kimberly A Leonhard, Erik M McIntire & Karen D Montgomery

  2. Department of Statistics, University of Wisconsin-Madison, Madison, Wisconsin, United States

    Michael A Newton

  3. Research and Development, Life Technologies, Inc., Madison, Wisconsin, United States

    Tori L Sampsell-Barron

  4. Biotechnology Department, Madison Area Technical College, Madison, Wisconsin, United States

    Tori L Sampsell-Barron

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  1. Seth M Taapken
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  2. Benjamin S Nisler
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Correspondence to Karen D Montgomery.

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Supplementary Figures 1,2, Supplementary Tables 1—4 and Supplementary Methods (PDF 447 kb)

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Taapken, S., Nisler, B., Newton, M. et al. Karyotypic abnormalities in human induced pluripotent stem cells and embryonic stem cells. Nat Biotechnol 29, 313–314 (2011). https://doi.org/10.1038/nbt.1835

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